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KMID : 0624620080410070542
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BMB Reports
2008 Volume.41 No. 7 p.542 ~ p.547
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Transgenic tobacco plants overexpressing the Nicta; CycD3; 4 gene demonstrate accelerated growth rates
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Guo Jia
Wang Myeong-Hyeon
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Abstract
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D-type cyclins control the onset of cell division and the response to extracellular signals during the G1 phase. In this study, we transformed a D-type cyclin gene, Nicta;CycD3;4, from Nicotiana tabacum using an Agrobacterium-mediated method. A predicted 1.1 kb cyclin gene was present in all of the transgenic plants, but not in wild-type. Northern analyses showed that the expression level of the Nicta;CycD3;4 gene in all of the transgenic plants was strong when compared to the wild-type plants, suggesting that Nicta;CycD3;4 gene driven by the CaMV 35S promoter was being overexpressed. Our results revealed that transgenic plants overexpressing Nicta;CycD3;4 had an accelerated growth rate when compared to wild-type plants, and that the transgenic plants exhibited a smaller cell size and a decreased cell population in young leaves when compared to wild-type plants. [BMB reports 2008; 41(7): 542-547]
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KEYWORD
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CycD, Cyclin, Nicotiana tabacu, Nicta, CycD3, 4, Plant growth, Transgenic plant
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